< Back
1st Specialization in Medical and Pharmaceutical Biotechnologies
ECTS Credits | Number of Hours (h/year) | |
---|---|---|
3 | 30 | |
2 | 30 | |
2 | 30 | |
1 | 15 | |
5 | 75 | |
4 | 60 | |
4 | 60 | |
4 | 60 | |
4 | 60 | |
2 | 30 | |
3 | 45 | |
26 | 385 |
Biophysics and instrumentation in biotechnologies
ECTS Credits | 1 |
---|---|
Number of Hours (h/year) | 15 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0001/1 |
Prerequisite | Basis of experimental physics : newtonian mechanics, electromagnetism, waves. Notions of differential and integral calculus |
---|---|
Aims and Objectives | Understanding and discussions about instrumental techniques used in biotechnology. |
Description | Spectroscopic techniques of instrumental analysis: · Basis of instrumental analysis. · Mass Spectroscopy by TF. · Infrared spectroscopy by TF. · Nuclear magnetic resonance spectroscopy . Luminescence spectroscopy . . Protein crystallography by X-ray scattering. |
Bibliography | SKOOG D.A., HOLLER F.J & NIEMAN T.A., Principes d’analyse instrumentale, Ed. De Boeck Université. ATKINS P.W., Chimie physique, Ed. De Boeck Université. |
Teaching and Learning Methods | Lectures Group seminars or workshops Project or fieldwork visits |
Assessment Methods | Written examinations Projects or practical work Continuous assessment |
Notes | In part |
Language | French |
Animal and plant cell culture including laboratory
ECTS Credits | 5 |
---|---|
Number of Hours (h/year) | 75 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0002/1 |
Prerequisite | Knowledge of pro and eukaryotic cells, their physiology and biochemistry. Aseptic work, solution preparation, microscopy. |
---|---|
Aims and Objectives | To learn fundamental techniques of cell culture , advantages and inconvenients in order to have the possibility to make the good choice when working on cultivated eukariotic cells. How to work in a laminair flow hood and use of incubator, Pasteur oven, liquid nitrogen tank... |
Description | Most usual techniques of cell culture in research laboratory as well as in large scale production. Basic techniques about cell cultures will be done on different cell-types, dispersion and disrumtion of tissues, monolayer culture techniques, propagation and scaling up of cultures (trypsinisation, cell counting, microscopic observation, cell freezing, cloning efficiency..), measurement of growth and viability. |
Bibliography | ADOLPHE M. et BARLOVATZ-MEINON G., Culture de cellules animales, méthodologie, applications, Ed. Inserm, 2003. FRESHNEY R.I., Culture of animal cells. A manual Basic technique, Alan Riss Inc., New York, 2005. Plant cell culture, a practical approach, Ed. By R.A.Dixon, 1995. |
Teaching and Learning Methods | Lectures Laboratory work |
Assessment Methods | Written examinations Continuous assessment |
Notes | Yes |
Language | French |
Molecular biology
ECTS Credits | 2 |
---|---|
Number of Hours (h/year) | 30 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0003/1 |
Prerequisite | Basic molecular biology . General biochemistry.
|
---|---|
Aims and Objectives | Knowledge of practical methods used in preparation, purification and analysis of nucleic acids.
|
Description |
|
Bibliography | SAMBROOK J. et al., Molecular cloning, Cold Spring Harbor Lab. Press, 1989. J.Etienne et E. Clauser, Biochimie génétique -Biologie Moléculaire, Masson 2004
|
Teaching and Learning Methods | Lectures |
Assessment Methods | Oral examinations |
Notes | In part |
Language | French |
Applied techniques to biotechnologies
ECTS Credits | 2 |
---|---|
Number of Hours (h/year) | 30 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0004/1 |
Prerequisite | Advanced knowledge in general microbiology, biochemistry, physics, mathematics and general biology. |
---|---|
Aims and Objectives | To master the student with fermentors of different volumes and their use in biotechnological productions, including scale-up experiments, optimisation and Dowstream processes. |
Description | Fermentations: Fermentors ( sterilization, oxygen transfer, Kla, agitation…) Different types of bioreactors. Captors in fermentation, regulation and automatisation. Dowstream process ( separation, purification, drying...)
|
Bibliography | BOUDRANT, CORRIEU et COULET, Capteurs et Mesures en Biotechnologies, Tec & Doc. Lavoisier, 1994. SCRIBAN R., Biotechnologie, Tec et Doc., Lavoisier, 1988. FRERE J.-M. et GERDAY C., Les méthodes de purification et d'analyse des protéines, Masson, 1994. |
Teaching and Learning Methods | Lectures Tutorials exercises. |
Assessment Methods | Written examinations Oral examinations Continuous assessment |
Notes | In part |
Language | French |
Applied techniques to biotechnologies
ECTS Credits | 2 |
---|---|
Number of Hours (h/year) | 30 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0005/1 |
Prerequisite | Advanced knowledge in biochemistry, physics, mathematics and general biology. |
---|---|
Aims and Objectives | To draw up : the general strategy to a protein purification and then step by step the details of the purification protocol. |
Description | Protein purification :
|
Bibliography | SCRIBAN R., Biotechnologie, Tec et Doc., Lavoisier, 1988. SCOPES R., Protein Purification : Principles and Practice, 3ème éd., Cantor, C.R. (ed.), Springer-Verlag, 1994. FRERE J.-M. et GERDAY C., Les méthodes de purification et d'analyse des protéines, Masson, 1994. |
Teaching and Learning Methods | Lectures Tutorials exercises. |
Assessment Methods | Written examinations Oral examinations Continuous assessment |
Notes | In part |
Language | French |
Enzymology including laboratory
ECTS Credits | 4 |
---|---|
Number of Hours (h/year) | 60 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0006/1 |
Prerequisite | General biochemistry course. |
---|---|
Aims and Objectives | To know the different enzymatic processes and the industrial applications. Application of enzymes in organic chemistry, in industry and in everyday life To master the techniques allowing the production and the purification of a microbial enzyme. To learn strategies of determination of kinetic parameters, to study a inhibition mechanism and to determine kinetics parameters. |
Description | General properties of the enzymes. Enzyme kinetics. Inhibition. Physical and chemist factors. Allosteric systems.Inactivation. Cofactors. Immobilised enzymes. laboratory: first part: production and purification of Escherichia coli galactosidase using lab-scale fermentors; different important parameters are followed during the process (cell concentration, enzymatic activity, protein concentration...); calculation of growth and production rates and purification yields. second part: to set up the optimal experimental conditions to the enzymatic study; the determination of the kinetics parameters; study of an inhibition process. Immobilisation of an enzyme and realisation of an enzymatic synthesis |
Bibliography | STRYER L. , Biochimie, Flammarion, Médecine-Sciences, 2008 FRERE J.M., Cours d'Enzymologie, University of Liège WONG & WHITESIDES, Enzymes in synthetic organic chemistry, Tetrahedron Organic Chemistry Series , Vol 12, Pergamon Press, 1995 DENEUVILLE F., Génie fermentaire, travaux pratiques, Ed. Doin, coll. Biosciences et techniques, France, 1991. SCRIBAN R., Biotechnologie, coll. Tec.et Doc., Lavoisier, Paris, France, 1988. THONART Ph., Cours de Microbiologie industrielle, Ulg. Revues : « Pour la science », « La Recherche », et « Biofutur », différents articles. |
Teaching and Learning Methods | Lectures Tutorials exercises |
Assessment Methods | Written and/or oral examination Continuous assessment |
Notes | In part |
Language | French |
Genetic engineering including laboratory
ECTS Credits | 4 |
---|---|
Number of Hours (h/year) | 60 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0007/1 |
Prerequisite | Molecular biology and introduction to genetic engineering.
|
---|---|
Aims and Objectives | Knowledge of genetic engineering procedures and their applications Practical approach of basic techniques in genetic engineering |
Description | Enzymes in use. Vectors and cloning strategies ( E. Coli, other microorganisms, yeast). cDNA and genomic libraries Recombinant protein synthesis. Directed mutagenesis. Genome mapping ( sequencing, gene capture). Gene therapy. Transgenic plants and animals. Medical applications. Laboratory : DNA isolation and purification Restriction Enzymes Electrophoresis Recombinant plasmid construction Transformation of E.coli cells Molecular hybridization Polymerase Chain Reaction |
Bibliography | SAMBROOK J. et al., Molecular cloning, Cold Spring Harbor Lab. Press, 1989. PRIMROSE S.et al., Principes de Génie Génétique, Ed. de Boeck, 2004 LONCLE et al, Génie génétique, Ed. Doin, 1993.
|
Teaching and Learning Methods | Lectures Tutorials Scientific papers analysis. |
Assessment Methods | Oral examinations Continuous assessment |
Notes | In part |
Language | French |
Immunology including laboratory
ECTS Credits | 4 |
---|---|
Number of Hours (h/year) | 60 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0008/1 |
Prerequisite | Hematology and Immunology courses. |
---|---|
Aims and Objectives | Analysis of the immune response ( natural and specific) in the normal situation and a few pathologic aspects |
Description | Natural immuno response. Specific immuno response. Inflammatory response. Maturation and differenciation of lymphoid B cells. Maturation and differenciation of lymphoids T cells. Cell-mediated. Cytotoxic response ( specific and NK). Antibodies response. Organ and hematopoietic transplantation. Cytokinines Vaccination. Hybridomas. HLA system |
Bibliography | MALE, Immunologie. Aide-mémoire illustré, De Boeck Université, 2ème éd.,1999. Manuel Qualité et ouvrages de référence du laboratoire d'hématologie du CHU, ULg. |
Teaching and Learning Methods | Lectures Tutorials |
Assessment Methods | Written examinations Oral examinations |
Notes | In part |
Language | French |
Applied microbiology including laboratory
ECTS Credits | 4 |
---|---|
Number of Hours (h/year) | 60 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0009/1 |
Prerequisite | Advanced knowledge in general microbiology, biochemistry, physics, mathematics and general biology |
---|---|
Aims and Objectives | The aim of the course is to train the student with fermentors of different volumes and their use in biotechnological productions, including scale-up experiments, optimisation and Downstream processes, |
Description | Introduction and history of industrial microbiology Recent examples of biotechnological products Industrial microorganisms (characteristics and taxonomy) Production of fermented food and drinks , of vaccines... Microbial growth, equations of batch, fed-batch and continuous cultures Optimisation of biotechnological and pharmaceutical productions Laboratory: Biomass production of an E.coli strain in different fermentation conditions (substrates, anae-/aerobiosis, fermentor/culture vessels ) and metabolites analysis e.g. in order to produce beta-galactosidase Investigation of the impact of different parameters ( cell density, substrate concentration, ...)on the success of the fermentation e.g. yields. Use of direct and indirect sensors and liquid/gas chromatography techniques.Investigation of Kla factor. |
Bibliography | LARPENT-GOURGAUD M. et SANGLIER J.J., Biotechnologies, Principes et Méthodes, Ed. Doin, Coll. Biosciences et Techniques, 1992. PRESCOTT, HARLEY et KLEIN, Microbiologie, Ed. De Boeck Université, 1995. SCRIBAN R., Biotechnologie, Coll. Tec et Doc., Lavoisier, 1988. THONART P., Cours de Microbiologie Industrielle, ULg. BOURDRANT, CORRIEU et COULET, CAPTEURS ET MESURES EN BIOTECHNOLOGIE (1994), Ed. Tec & Doc, Lavoisier. R. D. SCHMID (2005), Atlas de poche de BIOTECHNOLOGIE et de GENIE GENETIQUE, Ed. FLAMMARION DENEUVILLE F., Génie Fermentaire, Travaux Pratiques, Ed. Doin, Coll. Biosciences et Techniques, 1991. Mc NEIL B. et HARVEY L.M., Fermentation, a Practical approach, Oxford Univ. Press, 1990. Revues "Pour la Science", "La Recherche" et "Biofutur", différents articles www.genie-bio.ac-versailles.fr/ressources/ressources.htm
|
Teaching and Learning Methods | Tutorials Lectures with video illustration and slides. |
Assessment Methods | Oral examinations Continuous assessment Oral examinations and on-line documentation work ( internet). |
Notes | Yes |
Language | French |
Applied computer science
ECTS Credits | 3 |
---|---|
Number of Hours (h/year) | 30 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0012/1 |
Prerequisite | Basic knowledge of computer and Internet surfing Basic knowledge in genetics and molecular biology |
---|---|
Aims and Objectives | Practical knowledge of the most usual tools of biocomputing freely available on the Internet |
Description | Homology research Use of the tools necessary for the classical techniques of molecular biology . Philogenia Tertiary structures of proteins Microarrays |
Bibliography | |
Teaching and Learning Methods | Tutorials |
Assessment Methods | Projects or practical work |
Notes | No |
Language | French |
Placements
ECTS Credits | 26 |
---|---|
Number of Hours (h/year) | 385 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0010/1 |
Prerequisite | Theoretical and practical knowledge of the various matters |
---|---|
Aims and Objectives | To gain experience in one or another matter of the formation |
Description | Participation to fundamental or applied research projects , in university or biotechnological industry laboratories. |
Bibliography | |
Teaching and Learning Methods | Laboratory work Project or fieldwork |
Assessment Methods | Continuous assessment Report |
Notes | No |
Language | French |
Séminaires (y compris en anglais), visites d'entreprises biotechnologiques
ECTS Credits | 3 |
---|---|
Number of Hours (h/year) | 45 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0011/1 |
Prerequisite | Suffisant theoretical and practical knowledge of the curriculum disciplines Basic knowledge of scientific english |
---|---|
Aims and Objectives | To concretise courses by applications To be able to explain experimental procedures and results |
Description | Lectures and presentation of scientific articles in relation to biotechnologies. Participation to presentation and/or seminars (in outside laboratories) Visits of biotechnological and pharmaceutical companies |
Bibliography | |
Teaching and Learning Methods | Group seminars or workshops Talks |
Assessment Methods | Oral examinations Continuous assessment |
Notes | No |
Language | French English |
The information contained herein is intended for informational purposes only. The Higher Education Institution disclaims any responsibility with regard to its content.