1st Specialization in Medical and Pharmaceutical Biotechnologies
Genetic engineering including laboratory
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ECTS Credits | 4 |
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Number of Hours (h/year) | 60 |
Teaching staff | [donnée non visible pour l'instant] |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_SGBM0007/1 |
Prerequisite | Molecular biology and introduction to genetic engineering.
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Aims and Objectives | Knowledge of genetic engineering procedures and their applications Practical approach of basic techniques in genetic engineering |
Description | Enzymes in use. Vectors and cloning strategies ( E. Coli, other microorganisms, yeast). cDNA and genomic libraries Recombinant protein synthesis. Directed mutagenesis. Genome mapping ( sequencing, gene capture). Gene therapy. Transgenic plants and animals. Medical applications. Laboratory : DNA isolation and purification Restriction Enzymes Electrophoresis Recombinant plasmid construction Transformation of E.coli cells Molecular hybridization Polymerase Chain Reaction |
Bibliography | SAMBROOK J. et al., Molecular cloning, Cold Spring Harbor Lab. Press, 1989. PRIMROSE S.et al., Principes de Génie Génétique, Ed. de Boeck, 2004 LONCLE et al, Génie génétique, Ed. Doin, 1993.
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Teaching and Learning Methods | Lectures Tutorials Scientific papers analysis. |
Assessment Methods | Oral examinations Continuous assessment |
Notes | In part |
Language | French |
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