HEPL

General biochemistry course.

To know the different enzymatic processes and the industrial applications.

Application of enzymes in organic chemistry, in industry and in everyday life

To master the techniques allowing the production and the purification of a microbial enzyme.

To learn strategies of determination of kinetic parameters, to study a inhibition mechanism and to determine kinetics parameters.

General properties of the enzymes.

Enzyme kinetics.

Inhibition.

Physical and chemist factors.

Allosteric systems.Inactivation.

Cofactors.

Immobilised enzymes.

laboratory:

first part: production and purification of Escherichia coli galactosidase using lab-scale fermentors; different important parameters are followed during the process (cell concentration, enzymatic activity, protein concentration...); calculation of growth and production rates and purification yields.

second part: to set up the optimal experimental conditions to the enzymatic study; the determination of the kinetics parameters; study of an inhibition process.

Immobilisation of an enzyme and realisation of an enzymatic synthesis

STRYER L. , Biochimie, Flammarion, Médecine-Sciences, 2008

FRERE J.M., Cours d'Enzymologie, University of Liège

WONG & WHITESIDES, Enzymes in synthetic organic chemistry, Tetrahedron Organic Chemistry Series , Vol 12, Pergamon Press, 1995

DENEUVILLE F., Génie fermentaire, travaux pratiques, Ed. Doin, coll. Biosciences et techniques, France, 1991.

SCRIBAN R., Biotechnologie, coll. Tec.et Doc., Lavoisier, Paris, France, 1988.

THONART Ph., Cours de Microbiologie industrielle, Ulg.

Revues : « Pour la science », « La Recherche », et « Biofutur », différents articles.

exercises