1st Specialization in Medical and Pharmaceutical Biotechnologies

Applied techniques to biotechnologies

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ECTS Credits2
Number of Hours (h/year)30
Teaching staff[donnée non visible pour l'instant]
Course UnitCompulsory
TermQuad. 1 and 2
CodePARA-D_SGBM0005/1
Prerequisite

Advanced knowledge in biochemistry, physics, mathematics and general biology.

Aims and Objectives

To draw up : the general strategy to a protein purification and then step by step the details of the purification protocol.

Description

Protein purification :

  • Strategies for protein purification.
  • Detection and assay methods
  • Making crude extract
  • Electrophoresis methods
  • Chromatography methods
  • Exercices on informatics support.
Bibliography

SCRIBAN R., Biotechnologie, Tec et Doc., Lavoisier, 1988.

SCOPES R., Protein Purification : Principles and Practice, 3ème éd., Cantor, C.R. (ed.), Springer-Verlag, 1994.

FRERE J.-M. et GERDAY C., Les méthodes de purification et d'analyse des protéines, Masson, 1994.

Teaching and Learning MethodsLectures
Tutorials

exercises.

Assessment MethodsWritten examinations
Oral examinations
Continuous assessment
NotesIn part
LanguageFrench

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